Pseudotime estimation: deconfounding single cell time series

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Pseudotime estimation: deconfounding single cell time series

MOTIVATION Repeated cross-sectional time series single cell data confound several sources of variation, with contributions from measurement noise, stochastic cell-to-cell variation and cell progression at different rates. Time series from single cell assays are particularly susceptible to confounding as the measurements are not averaged over populations of cells. When several genes are assayed ...

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Slingshot: Cell lineage and pseudotime inference for single-cell transcriptomics

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LEAP: constructing gene co-expression networks for single-cell RNA-sequencing data using pseudotime ordering

Summary To construct gene co-expression networks based on single-cell RNA-Sequencing data, we present an algorithm called LEAP, which utilizes the estimated pseudotime of the cells to find gene co-expression that involves time delay. Availability and Implementation R package LEAP available on CRAN. Contact [email protected]. Supplementary information Supplementary data are available at Bioinf...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2016

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btw372